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Thermocycling conditions were as follows: initial denaturation at 93 for 3' followed by 40 cycles of 15 s at 95 30 s at annealing temperatures (which varied from 50 to 60 depending on the locus, see Supplementary Data of Table 2), extension of 30 s. http://ltzfc.com/comment/html/?24543.html  http://theartistexchange.net/profile.php?id=34690  This is nicely put! !